Part:BBa_K5233022:Experience

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Applications of BBa_K5233022

In our application, we design to express this basic part in E. coli, and our designed expression pathway is shown as follow :

This is the plasmid map of turboPETase(OmpA):

After the construction of the plasmid, we successfully express the engineered E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol

The results of the enzyme activity is shown as follows:

User Reviews

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